HB Imapanga Paradigm Yatsopano Yosungira Zitsanzo Zachilengedwe ku ICL

Imperial College London (ICL) ili patsogolo pa kafukufuku wa sayansi ndipo, kupyolera mu Dipatimenti ya Immunology ndi Kutupa ndi Dipatimenti ya Sayansi ya Ubongo, kafukufuku wake amayambira ku rheumatology ndi hematology mpaka ku dementia, matenda a Parkinson ndi khansa ya muubongo. Kuwongolera kafukufuku wosiyanasiyana wotere kumafuna malo apamwamba kwambiri, makamaka posungirako zitsanzo zofunika kwambiri zamoyo. Neil Galloway Phillipps, Senior Lab Manager m'madipatimenti onsewa, adazindikira kufunikira kwa njira yosungira bwino komanso yokhazikika ya cryogenic.

Zofunikira za ICL

1.Dongosolo lamphamvu kwambiri, lophatikizana lamadzi osungira nayitrogeni

2.Kuchepetsa kugwiritsa ntchito nayitrogeni komanso ndalama zogwirira ntchito

3.Kupititsa patsogolo chitetezo chazitsanzo ndi kutsata malamulo

4.Kupeza kotetezeka komanso kothandiza kwa ofufuza

5.Yankho lokhazikika lothandizira zoyambitsa zobiriwira

Mavuto

Dipatimenti ya Immunology ya ICL m'mbuyomu idadalira 13 osiyana static liquid nitrogen (LN).₂) akasinja osungiramo zitsanzo zoyeserera zachipatala, ma cell a satelayiti ndi zikhalidwe zoyambira zama cell. Dongosolo logawikali lidatenga nthawi kuti lisungidwe, likufuna kuyang'anitsitsa nthawi zonse ndi kuwonjezeredwa.

Neil anati: “Kudzaza matanki 13 kunatenga nthawi yochuluka, ndipo kusunga zonse kunali kovuta kwambiri. "Zinali zovuta, ndipo tinkafunikira njira yabwino yoyendetsera zinthu zathu."

Mtengo wokonza matanki angapo unalinso vuto lina. LN₂Kumwa kunali kochulukira, zomwe zinapangitsa kukwera kwa ndalama zoyendetsera ntchito. Panthawi imodzimodziyo, kukhudzidwa kwa chilengedwe ndi kuperekedwa kwa nayitrogeni pafupipafupi kunali kosemphana ndi kudzipereka kwa labu pakukhazikika. "Takhala tikugwira ntchito kuti tipeze mphotho zosiyanasiyana zokhazikika, ndipo tinkadziwa kuti kuchepetsa kugwiritsa ntchito nayitrogeni kungapangitse kusiyana kwakukulu," adatero Neil.

Chitetezo ndi kutsatira zinalinso zofunika kwambiri. Ndi matanki angapo omwe adafalikira kumadera osiyanasiyana, kutsatira njira zopezera ndi kusunga zolemba zamakono kunali kovuta. "Ndikofunikira kuti tidziwe bwino omwe akupeza zitsanzo, komanso kuti zonse zisungidwe moyenera mogwirizana ndi malamulo a Human Tissue Authority (HTA)," adatero Neil. “Dongosolo lathu lakale silinafewetse zimenezo.”

Yankho

ICL inali kale ndi zida zingapo zochokera ku Haier Biomedical - kusungirako kuzizira, makabati oteteza zachilengedwe, CO₂zofungatira ndi ma centrifuges - kukulitsa chidaliro mu mayankho a kampani.

Neil ndi gulu lake adalumikizana ndi Haier Biomedical kuti athandizire kuthana ndi zovuta zatsopanozi, kukhazikitsa CryoBio 43 LN yayikulu kwambiri.₂biobank kuti aphatikize akasinja onse 13 osasunthika kukhala njira imodzi yabwino kwambiri. Kusinthaku kunali kosasunthika, ndi gulu la Haier lomwe limayang'anira kukhazikitsa ndi kuphunzitsa ogwira ntchito ku labu. Dongosolo latsopanoli lidalowa mu LN yomwe ilipo₂malo okhala ndi zosintha zazing'ono zokha. Ndi dongosolo latsopanoli, kusungirako zitsanzo ndi kasamalidwe kameneka kwakhala kothandiza kwambiri. "Ubwino wina wosayembekezereka unali kuchuluka kwa malo omwe tidapeza," adatero Neil. "Ma tank onse akale atachotsedwa, tsopano tili ndi malo ochulukirapo mu labu la zida zina."

Kusintha kwa gawo losungirako nthunzi kwawonjezera chitetezo komanso kugwiritsa ntchito mosavuta. "M'mbuyomu, nthawi iliyonse tikakoka choyikapo mu thanki yamadzimadzi, imakhala ikudontha nayitrogeni, zomwe nthawi zonse zinali zodetsa nkhawa.

Neil ndi gulu lake adapeza kuti dongosololi ndi losavuta kugwiritsa ntchito, ndi pulogalamu yophunzitsira ya Haier yomwe imawathandiza kuti azitha kugwiritsa ntchito mwachangu.

Chomwe chinali chosayembekezereka koma cholandirika chinali njira zongobweza zokha, zomwe zimapangitsa kuti kulowa mu thanki kukhale kosavuta. "Ndi akasinja akale, ochita kafukufuku nthawi zambiri amayenera kunyamula zinthu zitatha." Ngakhale kuti thanki yatsopanoyo ndi yayitali, masitepe amayikidwa pakadina batani, zomwe zimapangitsa kuwonjezera kapena kuchotsa zitsanzo kukhala kosavuta kuwongolera," adatero Neil.

Kusunga zitsanzo zamtengo wapatali

Zitsanzo zomwe zasungidwa mu malo a cryogenic a ICL ndizofunika kwambiri pakufufuza kosalekeza. "Zitsanzo zina zomwe timasunga sizingalowe m'malo," adatero Neil.

"Tikukamba za kukonzekera kwa maselo oyera a magazi kuchokera ku matenda osowa, zitsanzo zachipatala, ndi zipangizo zina zomwe ndizofunikira pa kafukufuku. Zitsanzozi sizimangogwiritsidwa ntchito mu labu; zimagawidwa ndi ogwira nawo ntchito padziko lonse lapansi, zomwe zimapangitsa kuti kukhulupirika kwawo kukhale kofunika kwambiri. Kutheka kwa maselowa ndi chirichonse. Ngati sanasungidwe bwino, kafukufuku amene amathandizira akhoza kusokonezeka. Mtendere wa m'maganizo Titha kuyang'ana mbiri ya kutentha nthawi iliyonse, ndipo ngati titafufuzidwa, tikhoza kusonyeza molimba mtima kuti zonse zasungidwa bwino.

 Kupititsa patsogolo kukhazikika komanso kuwononga ndalama

Kukhazikitsidwa kwa biobank yatsopano kwachepetsa kwambiri kugwiritsa ntchito nayitrogeni mu labu, ndikudula kakhumi. "Iliyonse mwa akasinja akale amenewo anali ndi malita 125, kotero kuwaphatikiza kwapanga kusiyana kwakukulu," adatero Neil. "Tsopano tikugwiritsa ntchito kachigawo kakang'ono ka nayitrogeni tidachita kale, ndipo ndichopambana kwambiri pazachuma komanso chilengedwe."

Pokhala ndi zochepa zoperekera nayitrogeni zomwe zimafunikira, kutulutsa kwa kaboni kwachepetsedwa, kuthandizira zolinga zokhazikika za labu. "Sikuti ndi nayitrojeni wokha," adatero Neil. "Kukhala ndi zochepa zonyamula kumatanthauza kuti magalimoto ochepa pamsewu, ndipo mphamvu zochepa zomwe zimagwiritsidwa ntchito popanga nitrogen." Kusintha kumeneku kunali kofunika kwambiri kotero kuti Imperial inalandira mphoto zokhazikika kuchokera ku LEAF ndi My Green Lab poyamikira zoyesayesa zake.

Mapeto

Haier Biomedical's cryogenic biobank yasintha mphamvu zosungira za ICL, kuwongolera bwino, chitetezo ndi kukhazikika kwinaku akuchepetsa kwambiri ndalama. Ndi kutsata bwino, chitetezo chazitsanzo chowonjezereka komanso kuchepa kwa chilengedwe, kukwezako kwakhala kopambana kwambiri.

Zotsatira za Ntchito

1.LN₂kugwiritsa ntchito kuchepetsedwa ndi 90%, kuchepetsa mtengo ndi mpweya

2.Kutsata kwachitsanzo koyenera komanso kutsatira kwa HTA

3.Kusungidwa kotetezeka kwa gawo la nthunzi kwa ofufuza

4.Kuchulukitsa kosungirako mu dongosolo limodzi

5.Kuzindikiridwa kudzera mu mphotho zokhazikika